Generator

Part:BBa_K1230011:Design

Designed by: Andrés Ferriño Iriarte   Group: iGEM13_Ciencias-UNAM   (2013-09-25)


araC-pBad-rbs-LL37


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1164
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 999
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 981


Design Notes

In the absence of arabinose, AraC protein represses the structural gene by binding to araI1 and araO2 and the DNA forms a loop.


Source

2013 DNA distribution.


References